All the sytem objects & JCO connections not working in Portal

Hi,
Can any body tell me,
Last we we had copied the client copy from EIK 100 ( Production R3 server ) to EIT 900( Testing R3 server). From that movement.All the System object which are created in portal server to access the EIT 900 in R3 servers not giving the data .Even the JCO connections which are used to connect the the EIT 900 is not giving the data.
So , cany any body have idea where we need to check , how to trouble shoot this problem.
Regards
Vijay

Hi Brad,
Thanks for the reply.
Can u please tell me , both the servers are have the sld connection.
SLD connections are in the following setup.
TestServer(Portal) ---> EIT900(R3 Test Server)
Production(Portal) --->EIK100(R3 Production Server)
Can u please tell where will be the problem how i need to trouble shoot.
i have added one image of the JCO connections, all the connections got disabled. wht might be the reason.
I have added the bitmap of JCO connections in the following path for your reference.
JCO connections disabled.gif
Regards
Vijay

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All the sytem objects & JCO connections not working in Portal

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