Exchange 2007 wouldn't send out messages

I've have a 2007 Exchange server on a SBS 2008.
Yesterday IPv6 was deactivated, then we didn't recieved any mails. but now when it is activated again we can't send mail.
Nothing in the error log on the Clients. All i can find out is in Application log:
The Microsoft Exchange Mail Submission Service is currently unable to contact any Hub Transport servers in the local Active Directory site. The servers may be too busy to accept new connections at this time.
Event ID 1009
I feel i have tried everything, but it do not work. Any Suggestions?

Hi ,
I would like to know the answer for the queries.Please share me .
1.what happens when the user tries to an send an message ? Will that message is stucked in outbox else it was gone from the outbox ?
2.In case it gone from the outbox , can you able to see those messages on the queue viewer ?
3.Then What would be the error message on the queue viewer ?
4.Just try to telnet the hub transport server from your mailbox server and then try to send an test mail.In case if would have face any error messages please share me that ?
5.Please share me the output for the below mentioned command
get-transportagent  | ft -au
6.Please Share me the error/critical/warning events which is related to transport service on the hub transport servers .
How do I check CPU and RAM utilization on the transport servers?
If you go the task manager in hub transport server , you can able to find the cpu and ram utilization is
under normal or abnormal.
Thanks & Regards S.Nithyanandham

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