Experiencing same issues when running a seq file on different machines

I have a sequence file that when ran on a desktop PC, takes about 30 mins to run and the same file runs in about 3 mins on a laptop. All the station configuration options are same on both the teststands. The only difference I see between the two machines in running the test is that the desktop pc has 256MB of RAM and the laptop has 1GB of RAM. My question is, could this RAM difference be the reason for the 30 min to 3 min difference?? I am sure that all the other options inside teststand that can affect the speed at which the seq file runs are same on both the machines. Any suggestions/comments will be greatly appreciated.
Thanks
Anuj

Can you tell us a little bit more about what this sequence file does?  Does it communicate to instruments?  Does it access network resources (shared network drives)?
I am the founder of CnCSoftwareSolutions. When not cleaning up baby drool, I write about test data or work on Vision, a tool for understanding your test data. Visit me at www.cncsoftwaresolutions.com

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