Hue/Saturation not working?

I am working on a digital painting that was initially and grayscale, and I'm coloring it using hue/saturation, level, color balance, etc. adjustments. Until yesterday, this approach worked well, but when I go to adjust hue/saturation on any given layer it doesn't have any effect. This problem is not isolated to these works-- I also can't adjust it on other images including photographs. Can anyone help? I am in photoshop CS2 and in RGB mode, if that helps.
Thanks in advance.

Trash prefs.

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