ITunes 11 previously imported a video file, which I loaded on to my iPod Nano, but I removed the file from the iTunes library and now it won't (re-)import the same video. Any thoughts on why not?

iTunes 11 previously imported a video file, which I loaded on to my iPod Nano, but I removed the file from the iTunes library and now it won't (re-)import the same video.
I'm using 2 PCs, each with video files (.MP4) on their respective hard drives, and also one external drive with other video files on that I move between the two devices.
iTunes on one PC will (sometimes!) let me add video content from the external hard drive, which is clearly iTunes-compatible, because I can then copy it over to my iPod Nano. On the other PC, I couldn't load that same video from the external hard drive. But if I copied that same video on to the PC's internal hard drive first, then iTunes would let me import it. That was yesterday - now iTunes is refusing to load any files from the internal or external hard drive(s).
Any thoughts or suggestions, please?

So I tried copying the files from the external hard drive to a USB drive. I could import the video files from the USB drive!
So then I tried importing the same files on to my PC's internal hard drive again. I could import the video files from the internal hard drive!
So then I tried to import the very same files that failed to load on the very same PC previously. I could now import them!
I still can't import from the external hard drive directly, though.
I really don't think I like the process of importing things into iTunes very much... any suggestions on what's causing any of this behaviour would be very interesting.

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