Mail won't open either from the dock or from the applications folder. Icon bounces but program won't open

Mail worked fine this morning, then, when I went to open the program this afternoon, the icon bounced in the dock but would not boot either when I tried to open it from the dock or from the applications folder.

The nib file is the visual part of the user interface, so it has either got damaged or gone missing, which (unless you have been moving things around) is a little bit worrying.
I would suggest
make sure that you have a current backup
run disk utility to check your disk
if your disk is ok,
put in your installation disk and do an optional installation of Mail
download and run the latest combo upgrade

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