Question mark folder at startup, safe boot not working

Last night my iMac froze up, Force Quit didn't work, and I used the Power button to shut it off.  When trying to restart, it was getting stuck at the grey Apple.
I tried unplugging the peripherals and restarting; zapping the PRAM and restarting; safe boot and restarting.  Safe boot did it.
I then ended up going through those steps a few more times as my iMac starting freezing up again.  One of those times I changed all my applications in the dock that were set to launch at login to not do so, wondering if that was somehow causing a problem.  I also took a folder of recent items I'd created, mostly a LOT of PDFs of newspaper articles, burned it to a DVD and deleted the folder to free up more memory if that was part of the problem (I'm running out).  I then restarted from the Apple menu and it shut down through safe boot and then restarted without safe boot and did so just fine.  I ran Disk Utility and very few if any permissions needed repairing.  And it was operating OK for a bit and then froze up again.
Now I'm getting the question mark folder at startup and safe boot isn't working.  What should I be trying next?  Lion Recovery with Command-R?  I'm relatively new to Lion, but it had been working OK for me for a while now.
What other things should I try?  Would Target Disk mode possibly work from the MacBook I'm using now, which doesn't have Lion installed?

The question mark folder usually means that the
firmware could either not find a valid operating
system to boot, or, it could not find a hard disk
with an operating system on it.
Have you tried booting to Lion Recovery?  If
you can, then go into Disk Utility and do a
Repair Disk on your OSX volume.  If the
OSX volume is not showing up, you may need
to do a reformat and re-install.
Since it is a 24" iMac (from your description) it was
a pre-Lion Mac.  If you still have the original install
disks, try booting with it and use Disk Utility from it
to see if the hard drive is working.
Try this first before making a road trip.

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