Unable to backup iPhone 4 using iTunes 10.3.1.55

I have iTunes v10.3.1.55
iTunes says I have a bad/corrupt backup when I try to start the backup, but none are shown under Edit -> Preferences -> Devices tab -> Backups and the folder C:\Users\(username)\AppData\Roaming\Apple Computer\MobileSync\Backup\ [Vista machine] is empty!
Can someone suggest how do I go about fixing this?
Thanks,

Actually it's a good question ... I have not done it before manually. If the folder exists does it mean that I have done it before?
The folder is completely empty.  I reconfirmed that I have full-control persmissions on all folders below my username ...
C:\Users\(username)\AppData\Roaming\Apple Computer\MobileSync\Backup\ [Vista machine]
I have restricted access to the C:\User folder
- Read & Execute
- List folder content
- Read

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