743 download will not run - msg says it is "not a valid Win32 application"

We have ipod nanos which we use itunes with. We just got the newest nano which requires the 743 version, so I downloaded and saved it, but when I tried to run it, I got the message that it is "not a valid Win32 application". the new download is in my documents folder, but will not run. I tried going on to itunes to troubleshoot, but it told me to delete the download and try again. the only itunes I could find was the older version I am using. If I delete that, it will erase all my existing music, correct??? I DON'T want to loose my music again (been thru that when getting new computer). I did go to my documents and erase the 743 download in there, but when I tried to download it again, I got a message saying I already have it, and it shows up in my documents again.
PS I tried to back up my music on my flash drive, but it would not transfer to my new computer, so I have no backup. I need to install the new version to get music on my new nano. Any suggestions??? Getting frustrated. Thanks! Penny http://www.theallensisters.com

Have you tried the steps in this Knowledge Base article? See if it helps with that problem you're getting:
iTunes: Windows reports that iTunesSetup.exe is not a "valid Win32 application"
-Kylene

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