A/B tests are great - but what aspect is more important?

What aspects of a MKL you test most? Or, in other words, what is the most questioned question?
Is it the Subject line? Or maybe appearance? Sending time, your signature, addressing or something else?
I am asking mainly because the question of what we "could have done bettre" is always asked.
I read in https://blog.mailchimp.com/ab-testing-in-mailchimp-7-years-of-successful-experiments/ that the author tested the subject lines mostly, but I don't know if a good subject line is the selling trigger in a MKL.
What do you say? What do you think is the most important and what do test the most?
Thanks :-)
Ziv

Yes ziv subj line is the important factor and also the form submission also considered for analyzing the winning email...!!!! Thanks, gowtham

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