Adjustments Won't 'Stick'

Anyone ever come across the following :
PSE3 on Mac 10.5.6
Scanned b/w image
Layer New via copy
Blend Mode - any mode
Flatten image
Result - image goes back to unblended state.
Same problem with contrast adjustments etc.
I have reset the preferences/emptied the caches/repaired permissions.
The problem manifested itself today,seems to be confined to a small batch of recently
scanned tifs,and previously scanned images can be adjusted as normal.
The method of scanning hasn't changed, the files are not exceptionally large
- about 30Mb,and have the same attributes as any other file from the scanner.
I scan to a folder,and open the images from that photo folder.

Barbara....
I had tried moving it to the desktop previously,and it made no difference.
However,belatedly looking back at an answer you gave me when I had a problem
with save to web, I realised I had possibly not reset all the preferences.
I had reset the preferences using the keyboard,and had probably not reset
all of your three recommendations - Plist / Paths / Settings - I assume only
Settings had gone.
When all three had been deleted via Library,things appeared to return to normal.
I'm assuming the keyboard preferences reset doesn't cover Plist / Paths ?
There is still some very small reduction in the blending effect when I flatten the layers
- comparing the image with the layers unflattened to the flattened image,but that's probably
always been the case when dealing with grayscale images where it might be more noticeable.
Thank you for your patience,and apologies for wasting your time,when, If I'd read what you
said about my previous problem,I might well have sorted it out myself.
Hopefully the 80 or so forum members who have viewed this post might not make the same mistake I did.

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New member: My Settings won't stick; I don't know how to create a signature

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Message was edited by: Steve Kirk

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Quadcore G5 2.5Ghz Mac OS X (10.4.7) ENTOURAGE USER. AGAIN.

JF, I did understand you. It seems you've misunderstood me, though.
My desired arrangement:
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And that's what I've been using these 4 days without a hitch. My preferred arrangement is this:
Subject / From / To / Date
move on if you wish, I was kind of hoping for some fresh
insight - nothing personal
It seems you missed the smiley. I'm willing to figure out what's going on here, and that's the reason I've been trying to reproduce the problem. But I can't, that's all. I'm just wondering whether, according to your observations, I should leave the settings that way a few more days before giving up.
Also note that the -only- other column visible is the 'dot'
column (message status?) that you can't remove anyway.
In my case, I also have visible (both before and after making the change) Flags on the left and Size / Attachments on the right, i.e. my preferred arrangement is this:
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I clicked on the 'Drafts' mailbox, then back to the Inbox
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