After I remove the Block Diagram, The VI under RT target is always broken.

Why the VI is always broken under RT target if I remove their BD?
My LabVIEW Version is 2012 f1. I remerber that I have done a lot of remove in the last year and works well on RT. Really don't know why it's not work this time. Even for a very simple diagram...

I removed it's BD and put it under a PXI Real-time target. It says:
"This VI has a bad connection to or cannot find a subVI or external routine but it has no block diagram to show or fix the error. You must find or correct the subVI or external routine. Check for more information in the Explain dialog box in Get Info."

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