After intalling windows server 2008 R2 on database server, dstabase connections are very slow

Hi,
I upgraded my server from windows server 2003 to windows server 2008 R2. I have two application servers. Now applications on application servers are trying to execute queries but those are taking too long. It is very strange why it is happening.
Firewall rule is open for 1433 TCP and 1434 UDP.
Application servers are Windows Server 2003
Thanks in advance!
Thanks,
Sanjeev

Another possible cause is a problem with the application server.  You might try a reboot of the problem app server to see of that changes performance. 
If performance doesn't change after the reboot, try a coping a 100MB file from the database server to the application servers for performance comparison.  Assuming the performance difference for the copy is roughly the same as the queries, that
will eliminate SQL Server for troubleshooting and allow you to focus on networking as the likely culprit.  The fact that 10Mbps outperforms 100Mbps is concerning.  Perhaps the OS upgrade resulted in different drivers or interface configuration, or maybe
there is something else going on external to the servers, such as incorrect routing. 
I'm not a network guy but I would start by reviewing network interfaces on all servers to ensure they are configured properly and for full capabilities of the hardware and correct subnet and gateway.  Also review all components if your network
topology for proper configuration. 
For additional help with Windows networking, I suggest you start a new thread in the Windows Server Platform Networking TechNet forum (http://social.technet.microsoft.com/Forums/windowsserver/en-US/home?forum=winserverPN). 
Include the results of the file copy test and a link back to this thread.
Dan Guzman, SQL Server MVP, http://www.dbdelta.com

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