Application not working in Quality, which was working fine in Dev. Urgent!!

Hello Guru's,
We are experiencing a problem in portal.
Issue: We customized application in MSS( General Information which calls FM in the backend to populate the data) using NWDS and deployed the application into Development System and it's working fine. so we used NWDI to transport the code into Quality and the application is not working fine in Quality. The Issue is when transported the application into Quality it is not showing any data. and when checked the RFC trace it is not showing any FM been called when accessing the application. And also when checked in NWA it is not showing any error, when checked the JCo connections they are working fine.
Anyone please help us out. It's very Urgent.
Thanks in Advance.
Regards,
Pramod
'Points will be awarded for sure for helpful answers.'

Hello Harini,
Thank you for the reply, and I was expecting answer from you. Was seeing other forums and your replies to those .
Anyways Let me tell a little more on the issue:
We are implementing ESS/MSS in portal as per the req. we need to create a custom iview with employee details. And for that we have created a FM which will pick up the data and we are accessing that Function Module in WebDynpro application.
We deployed the code into QA and Prod In prod it is working fine but in quality it's giving 500 Internal Server Error
when checking the log file we are not getting any error logged. and checking in the R/3 whether that FM is executing when accessing the application using ST05 tcode it is not showing up the RFC trace either.
And Configuration of QA is same as Prod and all the permission are properly assigned.
Just wondering how is a application responding differently in different landscapes.
Looking forward for a solution.
Appreciate your time and help.
Regards,
Pramod

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