Authentication Problem with ACS 5.2 Using LDAP

HI!
I want to use LDAP for connecting to active directory but I get this Error from ACS 5.2 ( 22056 subject not found in the applicable identity stores).Is there anyone who can HELP me?
I used this configuration in ACS 5.2:
Users and Identity Stores / External identity store / ldap / Directory Organization
Subject ObjectClass : User
Subject Name attribute ; sAMAccountName
Group ObjectClass : Group
Group Map Attribute : MemberOf

Two questions:
- did you press "Test Bind to Server" from LDAP "Server Connection" tab and "Test Configuration" from "Directory Organization" tab?
- did you select the LDAP database as the result in the identity policy?

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IP Address: 10.2.12.45
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dot1x Failed over
mab Authc Success
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191527: .Jun 24 10:42:24.340 UTC: RADIUS: authenticator 7F A9 85 AB F6 4A D0 F3 - B4 E6 F2 56 74 C6 2D 33
191528: .Jun 24 10:42:24.340 UTC: RADIUS: NAS-IP-Address [4] 6 172.20.132.100
191529: .Jun 24 10:42:24.340 UTC: RADIUS: Calling-Station-Id [31] 19 "00:1F:29:7B:BD:82"
191530: .Jun 24 10:42:24.340 UTC: RADIUS: Acct-Terminate-Cause[49] 6 admin-reset [6]
191531: .Jun 24 10:42:24.340 UTC: RADIUS: Event-Timestamp [55] 6 1403606529
191532: .Jun 24 10:42:24.340 UTC: RADIUS: Message-Authenticato[80] 18
191533: .Jun 24 10:42:24.340 UTC: RADIUS: E0 3C B2 8C 89 47 67 A8 69 F5 3D 08 61 FF 53 6E [ <Ggi=aSn]
191534: .Jun 24 10:42:24.340 UTC: RADIUS: Vendor, Cisco [26] 43
191535: .Jun 24 10:42:24.340 UTC: RADIUS: Cisco AVpair [1] 37 "subscriber:command=bounce-host-port"
191536: .Jun 24 10:42:24.340 UTC: COA: Message Authenticator decode passed
191537: .Jun 24 10:42:24.340 UTC: ++++++ CoA Attribute List ++++++
191538: .Jun 24 10:42:24.340 UTC: 06D96C58 0 00000001 nas-ip-address(600) 4 172.20.132.100
191539: .Jun 24 10:42:24.349 UTC: 06D9AC18 0 00000081 formatted-clid(37) 17 00:1F:29:7B:BD:82
191540: .Jun 24 10:42:24.349 UTC: 06D9AC4C 0 00000001 disc-cause(434) 4 admin-reset
191541: .Jun 24 10:42:24.349 UTC: 06D9AC80 0 00000001 Event-Timestamp(445) 4 1403606529(53A95601)
191542: .Jun 24 10:42:24.349 UTC: 06D9ACB4 0 00000081 ssg-command-code(490) 1 33
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191548: .Jun 24 2014 10:42:26.353 UTC: %LINK-5-CHANGED: Interface GigabitEthernet0/3, changed state to administratively down
191549: .Jun 24 2014 10:42:27.359 UTC: %LINEPROTO-5-UPDOWN: Line protocol on Interface GigabitEthernet0/3, changed state to down
ISE-TEST-SWITCH#
191550: .Jun 24 2014 10:42:36.366 UTC: %LINK-3-UPDOWN: Interface GigabitEthernet0/3, changed state to down
191551: .Jun 24 10:42:40.592 UTC: AAA/BIND(000002A7): Bind i/f
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191553: .Jun 24 2014 10:42:42.580 UTC: %LINK-3-UPDOWN: Interface GigabitEthernet0/3, changed state to up
191554: .Jun 24 2014 10:42:43.586 UTC: %LINEPROTO-5-UPDOWN: Line protocol on Interface GigabitEthernet0/3, changed state to up
! SESSION ID CHANGES, USER ENTERS CREDENTIALS
! ERROR MESSAGE AT CLIENT "YOUR SESSION HAS EXPIRED"
! ERROR MESSAGE IN ISE "86017 SESSION MISSING"
ISE-TEST-SWITCH#show authentication sessions interface gi0/3
Interface: GigabitEthernet0/3
MAC Address: 001f.297b.bd82
IP Address: 10.2.12.45
Status: Running
Domain: UNKNOWN
Security Policy: Should Secure
Security Status: Unsecure
Oper host mode: multi-auth
Oper control dir: both
Session timeout: N/A
Idle timeout: N/A
Common Session ID: AC1484640000026C28C2FA05
Acct Session ID: 0x0000029D
Handle: 0x2C00026D
Runnable methods list:
Method State
dot1x Running
mab Not run

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