Best Throughput for RAID - Internal SATA vs. PCI RAID Card

Hi, we're upgrading our old server, which has been on an upgraded G4. We put 3 SATA drives in it, connected to a Highpoint 1820a RAID card running RAID 5.
We're moving this to an older G5 1.6 GHz model (2003). It currently has 2 internal SATA drive bays, but I know can be expanded with an adapter kit. It also has just plain PCI slots (not PCI-X or PCIe).
So I'm wondering what would provide better performance (throughput I guess):
• Using the 2 internal bays with a software RAID 1?
• Putting our old Highpoint RAID card in the PCI slot and running RAID 1 or possibly RAID 5 (with another drive)?
I know the RAID card doesn't use the processor for RAID functions, but I think in a RAID 1 it's fairly minimal, correct? I'm mostly wondering if the older basic PCI slot would bet the bottleneck in a fast system compared to the onboard SATA controllers?
THANKS!

Well, we're most concerned with data security and reliability. We're not streaming video or anything else that would demand extreme throughput. The server is mostly serving files to 4-5 users. So I imagine the performance gains would only be noticed when opening/closing files off the server, correct?
And we already own the Highpoint card, which has 8 internal SATA ports, so we'll probably use this. It works fine on PCI bus. So we can run this with 4 drives in either two RAID 1s (boot & files); a RAID 5, or a RAID 10.
We'll probably stick with Western Digital or Seagate 'enterprise' SATA drives, such as the WD RE3.
So, do you think we would see much real-world difference between the different RAID options (RAID 1 software, RAID 1 hardware, RAID 5 hardware, RAID 10 hardware)?
Thanks!

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