BizTalk AS2 Issue

Hi,
We have setup BizTalk AS2 and we are receiving the following error for the specific send port (on outbound):
The adapter failed to transmit message going to send port “” with URL “”.
It will be retransmitted after the retry interval specified for this Send Port. Details:"Unable to read data from the transport connection: The connection was closed.".
Note we are sending to the same URL for another sendport (and its working fine), but we are changing the AS2 ids (as one is for a canada and another is for US). Any direction would
be great. Thanks in advance.
System information:
Windows Server 2008 R2
BizTalk Server 2010
Thanks,
Jacob

Hi Jacob,
I understand that the same URL is working, but how about the party configuration and its association with the send port? Have you changed/updated the party when you change the URL? Check it.
If this answers your question please mark it accordingly. If this post is helpful, please vote as helpful by clicking the upward arrow mark next to my reply.

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BizTalk AS2 receive location (500.0 error) reported by eternal party when trying to reach BizTalk 2010.

Hi There -
We are facing AS2 connection problem with one of our customers. Following is scenario.
Problem Scenario –
We BizTalk 2010 server with which we are trying to connect one of our customer’s bank for secure communication.
Our UAT box has been done with a successful test connection with that bank however when we move to production server, whenever the Bank try to send any simple .txt file via AS2 protocol, we do not receive the file and bank receive an error following
message .
<div><fieldset>   <h2>500
- Internal server error.</h2>
<h3>There is a problem with the resource you are looking for, and it cannot be displayed.</h3>
Bank has AS2 setup on following environment.
AS2 Software Used
Connect Enterprise
AS2 Software Provider
Sterling Commerce (IBM Mail Slot)
Platform on which AS2 Software is installed
AIX
I have compared the UAT and Production servers AS2 receive URL configurations and these are completely alike.
Whenever I try browsing the virtual directory setup for AS2 from IIS 7.0 or from
internet explorer with hostname and virtual directory, I get 500.0 internal error which following post says is absolutely fine, this should not be a problem.
XXXXXXX
I have setup the IIS Log trace. Following are the traces from IIS.
Action Performed to resolve the issue.
Compared the UAT and Production server settings both are alike.
Setup the AS2 receive with reference of following URL .
http://msdn.microsoft.com/en-us/library/bb727975.aspx
http://biztalk-dish.blogspot.com.au/2012/07/guidance-on-edi-over-as2-in-biztalk.html
http://www.biztalkbill.com/Home/tabid/40/EntryId/66/Configuring-BTSHTTPReceive-dll-to-work-on-IIS-7.aspx
Installed the party certificates by referring following URL
http://msdn.microsoft.com/en-us/library/aa559902.aspx#step5
App Pool a/c and (Isolated and Inproc) host a/c and the receive pipline host a/c are same and certificates has been installed by loggin to server with same account .
Firewall ports are open to accept the traffic, I have to log from network team.
I tried following URL.
http://go4answers.webhost4life.com/Example/http-receive-url-without-78425.aspx (I have suffix the DLL name to the URL which party has to reach.
Example - http://XXXXXXX/EDIIN/BTSHTTPReceive.dll
http://hostname/virutalDirectory/BTSDLL
I have tried resolving 403/ 405 error which sometimes reported in my IIS error log, which generally arrive after reset of IIS 7.0
Using URL -
http://go4answers.webhost4life.com/Example/btshttpreceivedll-405-error-57131.aspx
http://biztalk-diary.blogspot.com.au/2010/05/btshttpreceive-error-405-method-not.html
IIS errors has been referenced using following Url
http://support.microsoft.com/kb/943891/en-us
following are the error logs from IIS 7.0 log file, whenever party sends any message to our server.
2013-12-14 19:02:59 10.83.241.83 POST /EDIIN/BTSHTTPReceive.dll - 80 - 10.83.251.4 Jakarta+Commons-HttpClient/3.0-rc4 500 0 0 9812
2013-12-14 19:03:48 10.83.241.83 POST /EDIIN/BTSHTTPReceive.dll - 80 - 10.83.251.4 Jakarta+Commons-HttpClient/3.0-rc4 500 0 0 343
2013-12-14 19:04:48 10.83.241.83 POST /EDIIN/BTSHTTPReceive.dll - 80 - 10.83.251.4 Jakarta+Commons-HttpClient/3.0-rc4 500 0 0 406
Sometimes I find following entries though
#Fields: date time s-ip cs-method cs-uri-stem cs-uri-query s-port cs-username c-ip cs(User-Agent) sc-status sc-substatus sc-win32-status time-taken
2013-12-14 20:10:55 ::1 GET /EDIIN - 80 - ::1 Mozilla/4.0+(compatible;+MSIE+8.0;+Windows+NT+6.1;+WOW64;+Trident/4.0;+SLCC2;+.NET+CLR+2.0.50727;+.NET+CLR+3.5.30729;+.NET+CLR+3.0.30729;+.NET4.0C;+.NET4.0E)
301 0 0 453
2013-12-14 20:10:55 ::1 GET /EDIIN/ - 80 - ::1 Mozilla/4.0+(compatible;+MSIE+8.0;+Windows+NT+6.1;+WOW64;+Trident/4.0;+SLCC2;+.NET+CLR+2.0.50727;+.NET+CLR+3.5.30729;+.NET+CLR+3.0.30729;+.NET4.0C;+.NET4.0E)
500 0 193 15
Any advice will be a great help.
Please Note: We have setup other virtual directories for receive HTTP connection using (BTSHTTPReceive.dll) file and these connections has been setup using same user account and are working perfectly fine.
I repeat,
>>           I have compared all the configurations for AS2 receive location with other setup on Production for other customers ( this is similar)
>>           I have cross checked the setting with UAT box where connection with this Bank is working absolutely fine but in production it is NOT and configurations are similar.

Hi,
It maybe windows firewall issue. you can try create a Windows Firewall exception if
Windows Firewall is running on your server. And please refer the blog about 500 error in iis which might helpful:
http://support.microsoft.com/kb/942031/en-us
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