Blank screen in silverlight web app (browser app)

I have a web server where to host my applications made in lightswitch with
silverlight client, I have several applications that are already running at more than
6 months without problems.
I did not update any of my applications lightswitch and not updated the server and did not install any programs.
Today I tried to open one application of several I have in this server and came across a white screen without showing the application logon screen, tried to access another application and the same thing happened, I tried to access another application and this
worked.
below has a video showing what happened
http://1drv.ms/1bwoAlS
I have done cleaning the silverlight my machine according to this article from microsofit,
https://support.microsoft.com/pt-br/kb/2608523 did a fresh install silverlight and tried to access the application again and it did not work, the same problem again
what is happening? I can not understand how something that is working perfectly, stops working suddenly without explanation or errors, I used fiddler and a look at the image below
Is not showing any error, what am I going to do? my clients are with this problem and do not know what to do to fix this, please help me solve this problem.
My machine
IE: 11.0.9600.17728
SL: 5.1.30514.0
SO: W7 Professional SP1 64bits
Thank you.

Hello Ivan,
Thanks for the help, I will try to do what you said
What types of permission you applied to everyone in wwwroot folder of IIS?
Thank you

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