Can I use my Mac to make a backup file of a MS DOS file

Can I use my Mac to make a backup file of a MS DOS file?

Have you verified that the mill is using FAT12?
Do I understand correctly that the mill boots from the floppy - it has no other storage?
If that is the case, then the new disk needs to be in the correct format and set up as a boot disk, which will not happen automatically, and I'm not sure if you can do it with OS X. The instructions on this page should help.
If it is something you need to do a lot of, and have no access to an MS-DOS computer, you could consider installing the Parallels virtual machine software on your Mac, and setting up a virtual MS-DOS system.

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