Can't open or move file - In use by Mac OS X

I have a file (movie) I am trying to move to a new external hard drive. I can't move it cause it says it is used by Mac OS X and cannot be moved or if I try to open it says it can't be opened.
The permissions are set correctly on this file and it plays just fine in iTunes but I can't open it in quicktime or I get that message. Anyone have any ideas to try? The Locked check box is not checked in the info window and my user has read and write access to the file.

Well I will never know what happened with that file. I tried moving it again and got the error message again. Got another message after that saying do you want to continue to move items you can move (though this was the only file I was trying) I said continue and then the file disappeared off my system.
No idea what happened with it.....

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