Cannot Uncheck CS3 Scratch DisK Selection Box

I'm running CS3 on a fairly recent 5 SATA hard drive XP machine. The drives are not raid arrays -- each drive partitioned into one or two partitions is seperate.
I presently have a 20GB Partition for my Photoshop Scratch Disk. The partition named "S" sits on a physical drive that does not contain Photoshop or my XP System Data.
I recently purchased a couple of 1TB drives. The new drives are faster then my present drives because of areal density. Therefor I've created a 20 GB partition on one of the new drives for a Photoshop Scratch Disk.
Finally here comes my question:
Going to Edit>Preferences>Performance I attempted to uncheck my old Scratch Disk as I no longer wanted it active. Photoshop will not allow me to do this. I can check & uncheck my new Scratch Disk. I can not boot into safe mode & attempt to uncheck the box as that terrible Flexnet Licensing does not allow Photoshop to run.
I can not reformat the "S" drive with Windows -- it appears Photoshop is preventing that operation.
Does anyone have a suggestion?
Thanks in advance!

"Just to cover the basics...you can't uncheck one scratch drive unless there's at least one other checked."
Oh No! -- I'm that STOOPID!
Freeagent -- thanks that was the problem. After activating my new scratch disk I could THEN UNCHECK my old one.
Thanks to both you & Michael for taking the time to help -- Best to both of you.

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