"Cannot unmount harddrive" Can you please offer help/advice?

Hi!
I run Tiger 10.4.11 on a first generation MacBook (Core 2 Duo) with a 160 GB HD split into 2 partitions. Using SuperDuper I made a bootable clone of my main partition on an external LaCie HD.
While booted from the clone on the external LaCie HD I tried to repartition the laptop's harddrive so that it will contain only one partition. The process failed several times due to this error: "unable to unmount partition 1".
I used DiskWarrior 4 to verify, repair and replace directories on both partitions of the laptop and everything seems to be fine according to DiskWarrior. Nevertheless, while booted from the external HD, one of the 2 partitions on the laptop still cannot be unmounted.
Can anyone offer any advice/suggestion?
Thanks a lot.
D.M.

Hi!
As promised, here is the update:
1. tried to switch off Spotlight...no effect.
2. tried to umount using terminal.... "could't unmount disk0...- error 49153
3. tried to unmount using original OS X disk1-->Disk Ulitily--> erased the partition--result: OK
Now, with the MacBook Hard Drive erased and partitioned to only one partition, I booted from the Clone on the external HD and copied this clone back to the MacBook harddrive.
So far so good... everything on the laptop works, etc.
Now, when I assign the Clone on the external HD ( let's call it: MacBook Clone) as startup disk and boot from it, the MacBook HD is again "unmountable" (which is expected since is a clone of the previous system).
The question therefore is: what is it that keeps MacBook HD "in use". How do I find out what are the active programs (perhaps hidden?) that contribute to this situation? I strongly believe this is the underlying problem since I have been cloning back and forth for years and never encountered anything similar.
Do you or anybody reading this post know where should I look or point me in the right direction?
Thanks a lot, beforehand.
D.M.
I have
Message was edited by: D.M.

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