CS4 Photomerge problem

I'm having trouble stitching photos together in CS4.  I've been searching for an answer online but all the problems I've encountered are different than mine.  I am stitching together photos from microscopic flourescence images.  I am trying to get a single image of the entire specimen but every time I try photomerge it leaves one of the images in the corner.  It does a great job of adding all of the other images together but every time it refuses to add the last image (it could be the first, the point is that it is an image on one of the ends).  I've tried this with several different sets of photos from my microscope and I always encounter this problem.
Thank you for your help.

It could be that the microscope lens has some distortion (pincushion or barrell) that throws alignment off. I don't know how you could test for (or correct) that in a microscope.
There is one way to "trick" photomerge (or auto-align, which I use mostly, but it's the same thing) into accepting images it otherwise wouldn't. Crop the adjacent image to include only the overlap, do the photomerge on those two and then put the resulting "aligned" image in with the rest. Nudge into place.
You could even do it as a two-step process, splitting the problem image into two, first the overlapping part and then the non-overlapping.
Make sure to lock the base (cropped) image so it doesn't get transformed.

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