Daisy-Chained G-Tech 400/800 and Mini

I've got a Mini that I use as my entertainment system (all music, video, TV, etc.)... I use a lot of harddrive space and currently have a G-Tech 500gb Firewire 400/800 drive that has one firewire 400 and two firewire 800 ports. I use this drive to store all my music, movies, TV shows, etc... and a second one to backup my Mini, my media external hard drive, and my MacBook Pro...
My question is as follows:
I know that with Firewire I can daisy chain the drives... however, my Mini does not have an 800 port... So, can I do the following without running into problems:
Mac Mini connected to "Media Drive" via Firewire 400 - connected to "Backup drive" via Firewire 800?
Basically my question is can some devices in a daisy chain be connected via 400 and others via 800 or does a daisy chain need to remain consistent using only 400 or only 800 throughout?
Otherwise, I guess I'll just use a hub... though this option rubs me the wrong way for some reason... it seems like I'd be sacrificing something by going through a hub... is this unfounded?
Mac Mini   Mac OS X (10.4.9)  

That's what I thought you meant. The issue is whether the drive in the middle has a cross-connection between FW 400 and 800 ports, and that is not likely (though technically possible despite the different specifications).
I would check with the manufacturer of the drive you intend to use in the middle of the chain to see if there is such a cross-connection on the FW 400 and 800 interfaces, but FW 400 and 800 aren't like USB 1.1 and 2.0 with their backwards compatibility.

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