Dll file is missing on installing the app-v 5.0 sequenced application

HI All,
I have sequenced a application using AppV 5.0 Sequencer. During sequencing the application is fine, I gave the PVAD as C:\Program Files (x86).
After installation of the sequenced application, on launching the shorcut it throws error that 
 "The program cannot start because PBVM100.dll file is missing from your computer. Try re-installing the application".
I searched for that dll file, the weird thing is the dll file is present in the ProgramFiles(x86), but still i dont why the application was throwing such an error.
If I install the source it is fine, but if I install the sequenced application it throws error.
I even tried connection groups through SCCM 2012, it is still throwing the same error.
I am out of ideas. Can anyone help me on this issue.
Thanks in advance.

Hello Cody. I created a sequenced
Actuate 7 eSpreadsheet Designer 10.0.2 with Appv-5 Sp2. I published the package using the Powershell commands, but When I tested the app in this test machine the app never openned. The process eSpreadSheet.exe cosumed the 99% of the CPU and never
open the app. I performed a Devirtualization in another machine and it looks work properly, So I think it's a virtualization problem. Do you have any idea how to fix this?
Note: I created another sequence including all the DLLS detected in SystemX86 in the same directory where the exe file resides for this application C:\Program Files\Actuate7 as you suggested in the last entry but I'm still having the same problem.

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