Error 50 during iTouch iOS 5 backup restore in windows vista

Hello!
I upgraded to iOS 5 recently. There wasn't enough space in c drive for back up. So I cut few program files and pasted in D drive. After the upgrade happened, I pasted the program files back to C drive.Then iTouch back up wasn't happening. It shows error 50.
Please help!!

Hi...
Click here > iTunes: Advanced iTunes Store troubleshooting
Then click: Specific Conditions and Alert Messagers
Then click: Error 50 ...

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