Error code -50 hard drives won't eject

I am using three G-drives, a 4TB RAID drive, and two 3-TB G-drives. On both my Macbook and iMac I am having problems ejecting them. I get the message
"The disk wasn't ejected because one or more programs may be using it." even when all the programs on the computer are closed.
I also get error code -50
"The operation cannot be completed. An unexpected error occurred (error code -50) when I try to create a new folder or move files on 2 of the drives.
At first I thought the problem was my iMac which recently had it's internal drive replaced, but I have been using the hard drives with my Macbook and am getting the same problems so now I think it's a problem with the drives, but since it is happening with all 3 I'm not sure what's up. I ran disk utility and verified the discs and it said everything was ok.
All three drives are used to store video files and FCP 7 media for editing.
I cannot find any information on what could be causing this and would appreciate any suggestions.
Thanks.

Welcome to Apple Discussions!
Usually this is indicative of a damaged directory. However, sometimes it is indicative of a dying hard drive,
or damaged peripheral interface.
Since you say all of your critical data is on the drives, I'd purchase Prosoft Data Rescue and another empty Mac formatted drive you can recover the data to and see if that is successful.
If that is not, follow my directory repair FAQ:
http://www.macmaps.com/directoryfaq.html
In the future always make sure you have at least two copies of your data at all times as my backup FAQ explains:
http://www.macmaps.com/backup.html

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