Error in Dreamweaver - File Permission Error?

I keep receiving this error in Dreamweaver CS6:
Started: 9/21/2012 12:01 AM
stylesheets\layout.css - error occurred - Access denied.  The file may not exist locally,  may be open in another program, or there could be a local permission problem.
File activity incomplete. 1 file(s) or folder(s) were not completed.
Files with errors: 1
Finished: 9/21/2012 12:01 AM
Sometimes the upload works, sometimes it doesn't.
What is going on here?

As an update I also had this issue in FileZilla but I get ConnectionTimeouts.
Just say this on the Status Page
Experiencing intermittent upload timeouts via FTP service on North America data center   20 minutes ago, Friday, September 21, 2012, 2, GMT-5:00 (PDT US Time)  Investigating reports of intermittent upload timeouts (NA DC related)

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