Error message "The document could not be saved. This file is already open" when trying to complete an electronic signature.

Running Adobe Acrobat Professional 10.1.0. I cannot upgrade to newer version because several applications were already tested on this version.
When trying to complete an electronic signature, my users receive the error message ""The document could not be saved. This file is already open".  When they close and open the file again to try to sign again they find a corrupted electronic signature.  Instead of a clean signature with the box colored in light blue they see their signature with the embedeed message Click Here to Verify & Sign with the box colored in grey.
We turned off antivirus in the Sharefolder where the forms are stored and nothing.  We turned off the antivirus on the client and nothing.  We stored the files in another server and nothing.  One workaround is saving the file in the desktop, signing it, and then back to the Sharefolder.  Another is saving the file with a different file name.
I've searched and the only similar thing was caused by the Windows Preview Pane.  See Information Transmogrification: Adobe Acrobat saving a file does not work, displays “File may be read-only”.  No luck with this one too.
Is definitively an intermittent problem.  The users that experience the problem experience it intermittently.
This started to happen when we move our computers to Windows 7.
Any ideas?

Correction on Question:
This is a web application and the users need to be able to load the editable .pdf form from the website, edit it and submit it back to the website from within Internet Explorer.
Users began getting the error message:
'The document could not be saved. This file is already open.'
last Friday after having no problems prior to this. Some users can edit the editable .pdf forms on the website and save the file with the same name to the website. Some get the error mentioned in the subject. 
We really need help getting this resolved.
Sandy Martin

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