Exchange Server 2010 - Authentication fails with DC

I have implemented Windows Server 2008 R2 Active Directory on Server: DC1
I have Implemented Exchange Server 2010 SP2 on Server: EMLSERVER
Previously MDeamon 5.5 was using for Messaging Services.
Manager told me he want only email server to be available after office hours.
For Exchange Server 2010 we have to power on two servers AD and EXCH.
For This reason i install AD on Exchange Server 2010 and Transfer all Roles from DC1 to EMLSERVER.
Now EMLSERVER is our AD and EXCH, and DC1 is our ADC.
To check this configuration i power off current ADC old DC (DC1).
when i try to test OWA, it was not working, and i get HTTP 500 Error, i try to troubleshoot it and finally after enabling Form Based Authentication Service HTTP 500 error has been resolved and i get OWA Page there when i try to Authenticate with Username
and Password i have got  HTTP 400 error and i am sucked now.
Can anyone suggest what should i do now.

The Requirement is that I have to provide a solution that is equivalent to MDeamon 5.5, where Company have just one DC which is use for authentication and group policy on Windows and another server which is MDeamon email server.
After office hours they power off DC and just email server is power on.
Therefore new solution must fulfill the same requirement, and in this scenario I have to put DC and Email together so that there is no power overhead.
1- Yes new DC which is together with Exchange is Global Catalog.
2- All Microsoft Exchange services are running.
3- There is no smart card authentication, there is just User/Pass require to authenticate.
4- Old DC/ Current ADC has been powered off after verifying transfer of all AD roles.
5- Previously I have installed DC on DC1 server and Exchange 2010 on EMLSERVER and it was working fine.

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    Netanel Ben-Shushan, MCSA/E, MCTS, MCITP, Windows Expert-IT Pro MVP. IT Consultant & Trainer | Website (Hebrew): http://www.ben-shushan.net | IT Services: http://www.ben-shushan.net/services | Weblog (Hebrew): http://blogs.microsoft.co.il/blogs/netanelb
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  • Need help with Outlook 2013 connecting to Exchange server(2010)

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    [email protected]
    [email protected]
    +918750003544

    Even I found the same issue once, and then I realised that the SP and RU should be same in exchange sever and the machine where the issue is.
    Could you please check the same if that  machine has the same Sp and RU, if not, please upgrade the same? I bet it will start working
    Regards,
    ASP20
    Please take a moment to Vote as Helpful and/or Mark as Answer where applicable. Thanks.

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