External Drive Backup and Partition Problems

I'd like to setup Time Machine to back up a smaller external drive (250 gigs) onto a larger external drive (1 TB). This is all I want to backup, nothing on the internal drive.
However, when I go to the exclude list, my smaller 250 gig hard drive is listed by default and I cannot remove it as it is "greyed out". Could this possible be because it's FAT32 formatted?
Additionally, I'd ideally like to format the 1 TB drive into 2 partitions - 1 to backup the 250 drive (so maybe like 400 gigs) and 1 to use for other things (600 gigs). I tried to do that with Disk Utility, but it keeps giving me an error and telling me to "try reducing the amount of change in the partition size".
If not that, is there any way to cap Time Machine's backup size otherwise? Sorry for the multipart question, let me know if you have any other ideas of how to make this work. Thanks!

Could this possible be because it's FAT32 formatted?
Yes. Time Machine can't back up a FAT32 drive.
(50585)

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