File Open By Another Application

I am trying to use an application to Sync files on 2 different IMACs across a Local Network. Many of the Syncs are failing because the Application claims that Files are exclusively Opened by another Application. I know that this is NOT true since I have NO other applications running. Is this an issue with the Application I am using to Sync the Files (ChronoSync) or is this a MAC OSX issue? Is there a recognized “Best” way to sync 2 IMACS both running OSX 10.8.3 across a Local Network?

Thanks for that Sarthak,
I don't have a USB drive but can confirm that I can save files when the file is on the mac desktop or on a USB stick without any issues.
So yes, at this point I think the issue is confined to Firewire drives (at least on my system which is 10.5.8).
Cheers
Ben

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