Help please with serila port i am going mad

hi all
i have onboard com port (serial port)
which dont work know (i dont know why)
so i buy this PCi Serial card
it was working very well untell now
but today i cant cominucate with my STB
its look like i can recive "signals" from device but
cant replay or send anything else
Recive=ok
send = not ok

big thx for ur reply
but i checked i/o on my bios
and its enabled
no there is no conflicts

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