Hi, I've lost all sound; keys F 3 4 and 5 only come up with a circle and diagonal bar across it. no sound in i-tunes and none in garage band or YouTube viewings

please help. all sound gone from my system. keys 3 4 and 5 only respond with line of bars and 'not permitted' circle with diagonal bar across. I've tried i-tunes garage band and YouTube. When I click on the loudspeaker icon at the top, I cant alter the slider below...

Could be many things, we should start with this...
"Try Disk Utility
1. Insert the Mac OS X Install disc, then restart the computer while holding the C key.
2. When your computer finishes starting up from the disc, choose Disk Utility from the Installer menu. (In Mac OS X 10.4 or later, you must select your language first.)
Important: Do not click Continue in the first screen of the Installer. If you do, you must restart from the disc again to access Disk Utility.
3. Click the First Aid tab.
4. Select your Mac OS X volume.
5. Click Repair. Disk Utility checks and repairs the disk."
http://docs.info.apple.com/article.html?artnum=106214
Then try a Safe Boot, (holding Shift key down at bootup), run Disk Utility in Applications>Utilities, then highlight your drive, click on Repair Permissions, reboot when it completes.
(Safe boot may stay on the gray radian for a long time, let it go, it's trying to repair the Hard Drive.)
Also, open Audio Midi Setup in Applications>Utilities, see the input & output options & KHz setting there, some things will change it for their own use, then not set it back.

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