How can i back up my wifes iPhone 3GS with my iTunes ?

I'm having problems trying to backup my wifes iPhone......
Basically, she has been using a 3GS that was my sons some years ago.....but now that my Daughter has upgraded, we have an iPhone 4 that she can start using instead.
However, when I connect to my iTunes, although it can see the 3GS, it doesnt give me any option to 'Backup', - which it did recently when I did the same exercise for my Daughters 'upgrade' and change of phone. (4 - 4S)
My iTunes is only giving me the options to 'set up as a new iPhone', or Restore from the Backups of either mine or my daughter's phones.
Is this to do with the software perhaps ?   Or does iTunes limit the number of handsets it will 'sync/backup' with ?
Any ideas/advice gratefully received......
Many thx .
Chris

Select setup as "New", the next screen should prompt you to rename your phone. Just name it what you already have it named. After that, you should be able to sync/backup and all of your data & settings will still be exactly as you've already set them up on your phone. That message is more threatening than what really happens.

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