How do I connect my drive to iMac for best transfer speeds using an adapter

Hi,
My question is: which port on my "old" LaCie hard drive should I use to connect to my iMac for optimum speed transfer? Should I use an adapter?
I am a pro music producer/songwriter & sound designer. I use large audio files in my AVID DAW Pro Tools 11 application both to read and write constantly.
For this I have dedicated hard drives such as a 6TB Promise Peagasus with Thunderbolt 1. But it's starting to run a little slow due to all the information residing only on this 1 unit.
I want to spread out my audio files on other hard drives as well. The one I MAYBE want to use, is a LaCie hard drive I have had for a couple of years.
Important: I only have 1 Thunderbolt port left to use on my iMac and 1 Ethernet port.
So do I need an adapter and what would be the speed then?
My question is how do I connect it for optimum speed transfer?
It is a LaCie D2 Quadra V2 E hard disk with 32mb cache.
It has:
1 FW 400,
2 FW 800,
1 High-Speed USB 2.0,
1 eSATA 3Gb/s.
I have an iMac 27" Mid 2011, 3.4 GHz Intel Core i7 with 16 GB 1333 MHz DDR3 running OS X 10.9.4.
I look forward to your replies.
Kind regards,
Michael

Thanks. Yes it is nice. Sounds interesting with your G force. I think I did put the Raid to 5, but cannot really remember. All I know is that when I did use Raid 5, I agreed to use 1 of the 6TB for some backup or security stuff that it takes care of on it's own. I thought it sounded good and safe when I installed it.
Am unsure how to check it.
I just read about the Raid block size which can be set to higher than my 32k in the Disk Doctor app to work faster with large amounts of continues data transfers. But it means that I should perform a new Raid. Not happy at all about this as I am very careful about my audio files.
Yes, I'll definitely go for the Thunderbolt/FireWire adaptor!

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