How to assign a layer as a output (Encode) alpha channel

Hi
In Adobe Premier or After Effects is there a way to assign a layer to be seen as a alpha channel on outputting (encoding) the completed project?
for examples.... I have about 6 video files all built up on top of each other (video 1, Video 2... and so on). The final layer (Video 7) I then import a PNG file with alpha to show the videos below where I want exactly. The black area (non transparent) area of my layer 7 I want to be seen as my alpha channel in my 3rd party video software after I have outputted (encoded) my video file.
Hope that makes thanks
Thanks
Craig

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Message was edited by: Steve Mussdorf

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How to assign a layer as a output (Encode) alpha channel

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