I am not able to read a barcode from a image

I want to read a barcode which is part of a big image on which i have to work on,i am not able to read the barcode as i have to give the correct ROI,which in my case has to be set dynamically,can any body help me in this

Please post an example image, and I'll try to help...
Copyright © 2004-2015 Christopher G. Relf. Some Rights Reserved. This posting is licensed under a Creative Commons Attribution 2.5 License.

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