Image sampling question

When working with composites, what the best method bringing in one image (300ppi) that will need to be reduced for the layout, into another file (300ppi). Will there always be a lose of pixel clarity due to the reduction or enlargement?

One other question with this all in mind, I'm creating a path for a a silo mask, should I
do it in the file that I'm bring the image into or create the path in the larger file and bring it in as
a vector mask? I'm assuming to do it once I've brought the image over since it might
be overkill on the path once I lose pixel res on the resized image?

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