Issues with itunes error -50, determining gapless playback, missing speaker options, home sharing not working

In the last few weeks I have been experiencing multiple issues with my iTunes.
I believe it began when my itunes library corrupted and I had to start again.
By starting again I mean I went to previous itunes libraries and used the most recent backup.
After I noticed a large number of unknown error (-50) messages I thought I better copy the backup I was using from the previous libraries folder over to the itunes folder (in case the error related to failed attempts to back up the library).
This problem in itself wasn't too bad as I am able to close and relaunch iTunes and carry on however I have noticed some less desirable side effects - the loss of home sharing. I am unable to pick speakers to broadcast to (I have 2 airport express units plugged in and working and an Apple TV2) and I am unable to access the iTunes library on Apple TV.
I've done some basic troubleshooting: creating rules to allow the home sharing ports, temporarily disabling antivirus/firewall, uninstalling antivirus/firewall, setting up rules on the router for homesharing ports, uninstalling iTunes, rebooting, reinstalling iTunes and retrying, disabling home sharing and reenabling, disabling peerblock, deauthorising itunes and reauthorising and rebooting both the pc and Apple TV, checking sharing settings under preferences and verifying both the entire library is shared and that the status of home sharing is on but no devices connected, created a new library and reimporting my media and the problem still exists.
I noticed that occasionally when I switch between libraries, deauthorise and reauthorise I do get access to home sharing and Apple Tv gets full access to the library BUT iTunes quickly becomes unresponsive and when I close and relaunch Apple TV can no longer see my library or iTunes.
I have checked the network I am running on is marked as a home network, I have tried connecting the Apple TV via LAN instead of WiFi but this makes no difference. What I do find interesting is that I have iTunes running on another pc and on a virtual machine and as soon as I run either their libraries appear on Apple TV so there must be something about the installation on my pc and nothing wrong with the Apple TV, airport express, router etc.
With that in mind I retried an uninstall and this time deleted as many tmp files as I could see in the iTunes library folder, rebooted and reinstalled. I also checked the permission levels on the iTunes application folder and the iTunes library folder (located on an additional internal drive), I then tried running iTunes as an administrator (which my user account is already set as) but all the problems are still evident.
The determining gapless playback has no effect on how I use iTunes but it clearly does not need to run for every single recently added track any time I make a minute change to other library entries. I tried looking up one of the files it was determining gapless playback for but it wasn't corrupt or read only so I don't see why it needs to retry continually.
I'm running Windows 7 64bit with all latest updates installed and iTunes 64bit 10.6.3.25, all apple devices are running the latest version of firmware and I'm running out of ideas. I did try running CCleaner to clear up and registry issues if there were any and I ran Appcleaner to remove any iTunes cache that may have been left between installs. I'm running out of ideas - what am I missing here?

I have found the problem was in part caused by Windows firewall.
Exactly how Windows firewall can cause the problem when I wasn't even using it I don't know.
What I do know is that when I checked 'allow a program through the firewall' I found 4 rules set for iTunes, one of which was public network access.
I deleted the superfluous rules and the Home Sharing problems are solved.
All that needs sorting now is the 'determining gapless playback'.

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