Itunes no longer loads

Vista Business
100gb hard drive
t7600 @2.33ghz
2gb ram
I get the error:
The iTunes application could not be opened. The file or directory is corrupted and unreadable.
I have partitioned my drive into 2 hard drives, I have run check disk on both and installed itunes on both drives. Itunes was working fine for weeks then all of a sudden the error started showing up. I have not installed any programs and have checked for spyware.

iTunes Library Genius.itdb is the broken file
# Recreate your iTunes Library
If you iTunes library has become corrupt, rebuilding it as described in "How to recreate your iTunes Library" may resolve the issue.
# Check for content files with issues
Sometimes a certain audio file can cause iTunes to quit unexpectedly or stop responding when it is analyzing gapless playback information. To determine which file(s) may be cause the issue follow the steps below:
1. Quit iTunes.
2. Move the iTunes Library file located at C:\Documents and Settings\username\My Documents\My Music\iTunes to your desktop
3. Open iTunes.
4. From the File menu, choose Add Folder to Library.
5. Navigate to the folder where your music is located. Typically this is
C:\Users\username\Music\iTunes\iTunes Music
6. Choose an artist, album, or single song to add the to the library
7. Wait for iTunes to successfully add the songs to your library and perform the gapless playback scan.
8. If successful, continue adding back more songs to your library.
9. If there is an issue with one of your files, you will notice your issue reappear after you attempt to add it to the library. At this point you should repeat these steps being careful not to add the files that cause the issue to occur.

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iTunes for Windows Vista or Windows 7: Troubleshooting unexpected quits, freezes, or launch issues
http://support.apple.com/kb/ts1717
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Itunes no longer loads

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