Layer position resets when creating animations

I'm creating an animated gif from a video clip (cinemagraph). In order to acheive the effect I need, I need to move each layer with the move tool to get an object to stay in the same position. I have gone through layer by layer and used the move tool to move the contents of each layer. When I play the animation I see the layers in the position I put them in. As soon as I stop playing the animation the contents of each layer snap back to their original positions. I have tried using the lock tools (both lock position and lock all) but they don't make a difference. Can anyone tell me why layer positions would reset when creating animations and how to get it to stop?
Thanks for any help.
Eric

It seems from your answer that you're thinking I'm just moving one frame and expecting the rest of them to move automatically (but I'm not sure if that's what you are saying). I have gone through and moved each frame individually and then locked each frame's position individually. When I play the animation and then stop it the layers move back to their original positions.
I'm also not sure if you're thinking I'm expecting that when I move one image off the layer it will affect the next layer. That's not what I'm expecting. I'm just repositioning the image on each layer to get a particular object to stay in one place (the camera was moving while shooting), so I'm dealin gwith each layer individually.
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