Lost everything, won't let me sync! help please!

I built a new comp a couple months ago, and have been able to sync successfully until about a week ago, when it started giving me the file not found error message. I've tried resetting comp, uninstalling a reinstalling itunes, restoring to original settings. When I try to sync now, it tells me it failed because backup session failed. then another window pops up saying that file could not be found.i do not use iphoto or mobileme. All of my downloaded apps still show up on the app tab when iphone is plugged in.
Thanks!

Are you getting 0xe8000065, unknown error -6999, or unknown error -48? It sounds like you have the P55 Intel chipset running Windows 7. If so there is a known issue with USB which stops communication with iPhone.
If so the solution is to update your BIOS.

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