Mail configuration in not working in Production.

Hi,
We are using SAP ECC6 with EHP 3 version with MSSQL2005 DB and WINDOWS2008r2 server.
For sending mails from SAP , we configured SCOT and SMTP configuration in all servers like DEV,QAS and PRD.
When i am sending mail from DEV and QAS its working fine, but in my PRD server mailis not going.and its giving below error message
Message cannot be transferred to node SMTP due to connection error (final) errior message number 816
all the configuration done as per the SAP standard document only, and its working fine in DEV and QAS.
below i am giving all the profile information and scot configuration ,SMTP port information and all..
Please check and help me to resolve this error.
This same parameters working in DEV and QAS...same parameters are there in PRD also.
Please check and help me.

Hi,
Check the connection from the SAP system to node SMTP. Perhaps the node is not assigned to the correct destination. If so, correct this in Customizing. In production operation, it is advisable to reroute provisionally to another node. You should therefore notify your system administrator.
XS816 error are likely caused by a problem with the connection between the SAP system and mail server.
It may happens due to the following, ( try to check port is open or not )
1. Check SMICM that ICM is running or not.
2. Check configuration with the help of note SAP Note 421359.
3. Check whether SMTP service/port are active and are correct.
4. Check these SAP Note 757804, 634006, 455140.
Thanks
KH

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Hi giovannizuccaro,
According to your description, when you configure database mail in your SQL Server Management Studio,
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For more information, there is detail about how to configure SQL Database mail so send emails using Office 365 (Exchange Online):
http://blogs.msdn.com/b/momalek/archive/2013/02/25/how-to-configure-sql-database-mail-so-send-emails-using-office-365-exchange-online-a-walkthrough.aspx
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