Mail inbox has mail but i can't open the mail box

My mail stopped working on my iMac, works fine on my iPhone iPad and in the iCloud. Now i have the inbox showing i have mail but it won't open,I've tried to use the Rebuild function but its greyed out. The only way i can see my emails is to log into the connection doctor and change a setting , then save and suddenly they appear until i click out to the other mail inbox.
I have noticed my signatures etc are missing so if i try to use one i get the following message
someone help please!

Writing your preferences to disk has failed. Your preferences may not have been saved. The permissions on ~/Library/Preferences may be wrong or your disk may be full.
this is the error message I'm on Lion 10.7.5

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