Mail lost all passwords and has taken all accounts offline

Using OS 10.9.4, Mail Version 7.3 (1878.6) and Keychain Version 9.0 (55153)
My mail went offline Saturday and I haven't been able to get back into it. I have verified access into all the accounts using Browser based access so have ruled out their being server issues etc.
Chrome kept asking me for passwords from keychain after the latest OS updates, I checked with Keychain and it stated that my Keychain file needed be repaired, so I selected repair, Chrome fixed and no longer asks. However now I cannot get into Mac Mail, all accounts are shown offline, it won't store any passwords and repeatedly asks me for passwords if I try and update or take the mailboxes on line.
If I go into Keychain I cannot unlock the keychain (ie click the padlock to unlock the login keychain)  and I have nothing showing..
Is there any known issues with keychain? I don't know enough about it to try things without risking breaking it...
Any suggestions gratefully received.
Graham

Back up all data.
Launch the Keychain Access application in any of the following ways:
☞ Enter the first few letters of its name into a Spotlight search. Select it in the results (it should be at the top.)
☞ In the Finder, select Go ▹ Utilities from the menu bar, or press the key combination shift-command-U. The application is in the folder that opens.
☞ Open LaunchPad. Click Utilities, then Keychain Access in the icon grid.
Select the login keychain from the list on the left side of the Keychain Access window. If your default keychain has a different name, select that.
If the lock icon in the top left corner of the window shows that the keychain is locked, click to unlock it. You'll be prompted for the keychain password, which is the same as your login password, unless you've changed it.
Right-click or control-click the login entry in the list. From the menu that pops up, select Change Settings for Keychain "login". In the sheet that opens, uncheck both boxes, if not already unchecked.
From the menu bar, select
          Keychain Access ▹ Preferences ▹ First Aid
If the box marked Keep login keychain unlocked is not checked, check it.
Select
          Keychain Access ▹ Keychain First Aid
from the menu bar and repair the keychain. Quit Keychain Access.

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