My iPhone no longer syncs after iOS 5.0.1 update.

I have an iPhone 3GS (16GB), and ever since I updated to iOS 5.0.1 it has been refusing to sync via USB and WIFI. Apparently it needs an additional 8.32GB of space, despite the fact that nothing's changed with regards to the content being synced. I've attached a screenshot for reference.
Thanks in advance for your help.

I had this issue too. I did an OTA update.
I tried turning off my iPhone, removed and reinserted my SIM and turning it on again a few times without success. I also inserted my wife's SIM, it was OK. When I tried with mine again, it was not. I also tried going in and out of Airplane Mode too without success.
Afte a bit of trial and error, I finally got it to work.
What I did was:
1. Turn off WIFI.
2. Turn off my iPhone.
3. Removed and reinserted my SIM.
4. Turned on my iPhone. Almost immediately I saw the "3G" indicator and after a couple of seconds, my SIM worked!
5. Turned WIFI back on.
From my issue, it seems the iOS 5.0.1 update got "confused" with the network setup.
HIH.
Andrew

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I had this issue too. I did an OTA update.
I tried turning off my iPhone, removed and reinserted my SIM and turning it on again a few times without success. I also inserted my wife's SIM, it was OK. When I tried with mine again, it was not. I also tried going in and out of Airplane Mode too without success.
Afte a bit of trial and error, I finally got it to work.
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5. Turned WIFI back on.
From my issue, it seems the iOS 5.0.1 update got "confused" with the network setup.
HIH.
Andrew

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Hi Sekhar_Nutakki,
Thanks for visiting Apple Support Communities.
I recommend these steps if your iPhone is shutting down or restarting unexpectedly:
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http://support.apple.com/kb/TS2802
Will not turn on, will not turn on unless connected to power, or unexpected power off
Verify that the Sleep/Wake button functions. If it does not function, inspect it for signs of damage. If the button is damaged or is not functioning when pressed, seek service.
Check if a Liquid Contact Indicator (LCI) is activated or there are signs of corrosion. Learn about LCIs and corrosion.
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The low-battery image appears, even after the phone has charged for at least 20 minutes: See "iPhone displays the low-battery image and is unresponsive" symptom in this article.
Something other than the Home screen or Low Battery image appears, continue with this article for further troubleshooting steps.
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Microsoft Windows XP Professional Service Pack 3 (Build 2600)
INTEL_ DX38BT__
iTunes 11.1.3.8
QuickTime 7.7.4
FairPlay 2.5.16
Apple Application Support 2.3.6
iPod Updater Library 11.1f5
CD Driver 2.2.3.0
CD Driver DLL 2.1.3.1
Apple Mobile Device 7.0.0.117
Apple Mobile Device Driver 1.64.0.0
Bonjour 3.0.0.10 (333.10)
Gracenote SDK 1.9.6.502
Gracenote MusicID 1.9.6.115
Gracenote Submit 1.9.6.143
Gracenote DSP 1.9.6.45
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Core Media is supported.
Video Display Information
NVIDIA Quadro FX 1500
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**** Device Connectivity Tests ****
iPodService 11.1.3.8 is currently running.
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