My login is showing server error when tryinng to login through my application

Server Error in '/' Application. Failed to generate a user instance of SQL Server due to a failure in copying database files. The connection will be closed. Description: An unhandled exception occurred during the execution of the current web request. Please
review the stack trace for more information about the error and where it originated in the code. SQLExpress database file auto-creation error: The connection string specifies a local Sql Server Express instance using a database location within the application's
App_Data directory. The provider attempted to automatically create the application services database because the provider determined that the database does not exist. The following configuration requirements are necessary to successfully check for existence
of the application services database and automatically create the application services database: If the application is running on either Windows 7 or Windows Server 2008R2, special configuration steps are necessary to enable automatic creation of the provider
database. Additional information is available at: http://go.microsoft.com/fwlink/?LinkId=160102. If the application's App_Data directory does not already exist, the web server account must have read and write access to the application's directory. This is
necessary because the web server account will automatically create the App_Data directory if it does not already exist. If the application's App_Data directory already exists, the web server account only requires read and write access to the application's
App_Data directory. This is necessary because the web server account will attempt to verify that the Sql Server Express database already exists within the application's App_Data directory. Revoking read access on the App_Data directory from the web server
account will prevent the provider from correctly determining if the Sql Server Express database already exists. This will cause an error when the provider attempts to create a duplicate of an already existing database. Write access is required because the
web server account's credentials are used when creating the new database. Sql Server Express must be installed on the machine. The process identity for the web server account must have a local user profile. See the readme document for details on how to create
a local user profile for both machine and domain accounts.

Hi migold,
As other post, please help to post more details for analysis.
Based on my research, the error “Failed to generate a user instance of SQL Server due to a failure in copying database files” is usually caused by that User Instance is set improperly. Please check for the existence of the "User Instance=True;" in
your connection string, if it exists, change it to "User Instance=False;". For more details, please review the following blog.
http://blog.benhall.me.uk/2008/03/sql-express-error-failed-to-generate-a-user-instance-of-sql-server/
Thanks,
Lydia Zhang
Lydia Zhang
TechNet Community Support

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