My usb ports dont remember whats in them!!

Hi, just got my computer back up and running and now the usb ports are forgetting what I have in them every time I restart or fresh boot my pc.  When in windows I have to take the usb connector out then reattach it and it detects it and runs.  I’m using the ports on my computer not a hub.  
They worked before on my old motherboard but not on my new one, it is a ms-6339 p4 1.7.
Any help will be appreciated.  

With your current setup, do you just have drive C: for Harddrives?
If yes, boot from startup disk and use CDROM support option.
Once it's booted, make sure you have access to your CDROM.
If you don't, you'd be better off finding someone there to help you.
At the A: Prompt type "format c:" without the quotation marks. Follow the directions.
Once formatted, put Windows ME CD in drive and change to that drive by typing in the drive letter at the DOS A: prompt.
Should be drive E: (Will say when you boot)
Type "setup" and Windows installation should begin.

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