Nano won't eject: files in use

Every time I try to eject from iTunes I get the message "cannot be ejected as it contains files that are in use by another application". I've tried to uncheck the "enable disk use" box on the summary page but it isn't active. Anybody know where I should go from here? Thanks L

I had similar frustrating issues with a MBP and an iPod Mini.  I think the issue was with Spotlight.  After unchecking "music" from the Spotlight search results, I've no longer had this issue.  You may want to give this a try:
Click on System Preferences and then Spotlight.  Uncheck the box next to "music".  Try to eject your iPod and see if it works.  Doing this prevents you from being able to search for songs from the Spotlight search box, but I'd rather deal with this than continually fight to get my iPod to eject. 
FWIW - I used to also try ejecting the iPod using Finder, but this only worked some of the time. 

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